| Burlington Northern Santa Fe Railroad | Alliance | 1300+ | Transportation |
| Box Butte General Hospital | Alliance | 295 | Healthcare |
| Parker-Hannifin | Alliance | 229 | Manufacturing |
| Alliance Public School | Alliance | 218 | Education |
| City of Alliance | Alliance | 110 | Municipality |
| Hemingford Public School | Hemingford | 104 | Education |
| WESTCO | Alliance | 97 | Agriculture |
| Highland Park Care Center | Alliance | 83 | Healthcare |
| Farmers Cooperative Elevator | Hemingford | 32 | Agriculture |
| Perrin Manufacturing | Alliance | 30 | Manufacturing |
| Western Potato Inc. | Alliance | 25 | Agriculture |
| Thompson Seed | Berea | 19 | Agriculture |
| Hemingford Telephone Co. / Mobius | Hemingford | 17 | Telecommunications |
| Simplot Growers | Berea | 15 | Agriculture |