| Burlington Northern Santa Fe Railroad | Alliance | 1500+ | Transportation |
| Box Butte General Hospital | Alliance | 290 | Healthcare |
| Parker-Hannifin | Alliance | 234 | Manufacturing |
| Alliance Public School | Alliance | 207 | Education |
| City of Alliance | Alliance | 113 | Municipality |
| Hemingford Public School | Hemingford | 107 | Education |
| Westco | Alliance | 97 | Agriculture |
| Perrin Manufacturing | Alliance | 43 | Manufacturing |
| Farmers Cooperative Elevator | Hemingford | 33 | Agriculture |