| Burlington Northern Santa Fe Railroad | Alliance | 1500+ | Transportation |
| Alliance Public Schools | Alliance | 307 | Education |
| Box Butte General Hospital | Alliance | 249 | Healthcare |
| Parker-Hannifin Corporation | Alliance | 247 | Manufacturing |
| WESTCO | Alliance | 230 | Agriculture/Transportation |
| City of Alliance | Alliance | 97 | Government |
| Hemingford Public School | Hemingford | 89 | Education |
| Perrin Manufacturing | Alliance | 60 | Manufacturing |
| Farmers Cooperative Elevator | Hemingford | 56 | Agriculture |
| Pepsi-Cola of Western NE | Alliance | 34 | Distribution |
| Progress Rail | Alliance | 30 | Transportation |
| Mobius Communications/ Hemingford Telephone Co. | Hemingford | 27 | Communications |
| Panhandle Rural Electric Membership Association (PREMA) |
Alliance | 25 | Rural Electric |
| American Electric Power | Alliance | 24 | Transportation |